Purification of his-tagged proteins in non-denaturing conditions suggests a convenient method for protein interaction studies
نویسندگان
چکیده
منابع مشابه
Purification of his-tagged proteins in non-denaturing conditions suggests a convenient method for protein interaction studies.
Studies of protein-protein interactions are of increasing interest in many areas of biological and biochemical research. Dimerization and other protein—protein interaction motifs that have recently been identified and shown to be important in many regulatory pathways include, for example, the leucine—zipper (1), helix-loop-helix (2), and SH2 and SH3 domains (3). Commonly used analytical methods...
متن کاملSynthetics of NiFe2O4 nanoparticles for recombinant His-tag protein purification
Recombinant protein purification is a kind of sensitive and expensive method in genetics engineering. Genetic manipulation leads to the expression of various proteins; it should be isolated with high purity finally. Differed methods for protein purification are categorized, based on cast, quality, Easy work and side effect of protein. In this article, we are investigating his His-tag protein pu...
متن کاملSynthetics of NiFe2O4 nanoparticles for recombinant His-tag protein purification
Recombinant protein purification is a kind of sensitive and expensive method in genetics engineering. Genetic manipulation leads to the expression of various proteins; it should be isolated with high purity finally. Differed methods for protein purification are categorized, based on cast, quality, Easy work and side effect of protein. In this article, we are investigating his His-tag protein pu...
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ژورنال
عنوان ژورنال: Nucleic Acids Research
سال: 1991
ISSN: 0305-1048,1362-4962
DOI: 10.1093/nar/19.22.6337